Introduction to Solid Supported Membrane Based Electrophysiology

Introduction to Solid Supported Membrane Based Electrophysiology

The electrophysiological method we present is based on a solid supported membrane (SSM) composed of an octadecanethiol layer chemisorbed on a gold coated sensor chip and a phosphatidylcholine monolayer on top. This assembly is mounted into a cuvette system containing the reference electrode, a chlorinated silver wire. After adsorption of membrane fragments or proteoliposomes containing the memb...

Functional characterization of a ClC transporter by solid-supported membrane electrophysiology

EcClC, a prokaryotic member of the ClC family of chloride channels and transporters, works as coupled H(+)/Cl(-) exchanger. With a known structure and the possibility of investigating its behavior with different biochemical and biophysical techniques, the protein has become an important model system for the family. Although many aspects of its function have been previously characterized, it was...

Separation of membrane-bound compounds by solid-supported bilayer electrophoresis.

Separation, purification, and detection of biomembrane species such as lipids and transmembrane proteins are difficult tasks. The processing conditions are often harsh, which can result in alteration of native structures or complete loss of material.1,2 Furthermore, it is difficult to detect subtle post-translational changes in these molecules that occur on the cell surface.3-5 Typical purifica...

Introduction to Solid State NMR

Introduction to Solid-phase Extraction

We have a sample in front of us. It is of unknown composition, but we know that it is complex, containing anywhere from a few hundred to many thousand chemical components. It is of uncertain physical form perhaps it looks like a liquid, but it has solid particles floating in it. We think that a particular compound is present in that sample, somewhere and in some form, but we don't know how much...